Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Medchemexpress LLC NaCl, KCl, Phosphate | AR/GR grade | 10 POUCHES
MCE PBS Powder (2 L of 1x) consists of NaCl, KCl, and Phosphate, and is widely used in immunoassay, microbiology, and protein experiments. The PBS buffer prepared from this product can be directly used for cell culture and other related experiments after being sterilized by 0.22 μm filtration. Each pouch can be diluted to 2 L of 1x PBS buffer, resulting in a concentration of 137 mM NaCl, 2.7 mM KCl, and 10 mM Phosphate. This product offers a convenient method for making PBS buffer, eliminating the need to weigh and mix individual components.
- Consists of NaCl, KCl, and Phosphate.
- Convenient way to make PBS buffer.
- Eliminates the need to weigh and mix individual components.
- All relevant raw materials are AR/GR grade.
- The pH of the 1x PBS buffer is 7.4 ± 0.2 @ 25°C.
Apexbio Technology LLC Annexin V-HF555/SYTOX Green Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid binding protein that specifically binds to phosphatidylserine (PS) PS is normally located on the inner leaflet of the cell membrane lipid bilayer but during early apoptosis PS is translocated from the inner leaflet to the outer surface of the cell membrane exposed to the extracellular environment Annexin V has a high affinity for PS and when labeled with fluorophore (HF555) Annexin V can serve as a fluorescent probe to detect apoptosis via flow cytometry or fluorescence microscopy The staining process requires only 10-20 minutes SYTOX Green specifically binds to double-stranded DNA emitting green fluorescence and normally cannot cross intact cell membranes However in late-stage apoptotic cells or dead cells SYTOX Green can penetrate the cell membrane and stain the nucleus By combining Annexin V-HF555 and SYTOX Green staining it is possible to distinguish among early apoptotic late apoptotic and dead cells
Biotang Inc Phosphate-Buffered Saline (PBS, 10X)For Western Blot Washing, 1000 ml
Phosphate-Buffered Saline (PBS, 10X)For Western Blot Washing, 1000 ml.
Boston Bioproducts Inc Saline-Sodium Citrate (20X) - 4L
Saline-Sodium Citrate (20X) - 4L (For Research Use and Further Manufacturing Only)
Electron Microscopy Sciences D-PBS, Formula III Phosphate Buffer Saline 1 LT
All of our Dullbecco's Phosphate Buffer Saline Solutions contain Potassium Chloride, Potassium Phosphate Monobasic, Sodium Chloride, Sodium Phosphate Dibasic, and D-Glucose. Some formulations may contain additional components. Solutions are prepared 1X strength.
FUJIFILM BIOSCIENCES INC MEM 1X Earles Salts w/o NEAA
MEM 1X Earles Salts w/o NEAA is an uncomplicated medium that is useful for a wide range of species and cells
Boston Bioproducts Inc Sodium Phosphate Buffer (0.2 M, pH 7.4) - 500ml
Sodium Phosphate Buffer (0 2 M pH 7 4) - 500ml (For Research Use and Further Manufacturing Only)
FUJIFILM BIOSCIENCES INC MEM 1X Earles Salts w/o NEAA
MEM 1X Earles Salts w/o NEAA is an uncomplicated medium that is useful for a wide range of species and cells
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
This DMEM-Hi glucose medium is a 1x complete medium with no added factors (common modifications) such as L-alanyl-L-glutamine HEPES or sodium pyruvate. It differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with L-glutamine or L-alanyl-L-glutamine.
Apexbio Technology LLC Annexin V-HF647/PI Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (HF647) can be used as a fluorescent probe to detect apoptotic cells through flow cytometry or fluorescence microscopy with staining taking only 10-20 minutes PI is a dye which specifically binds double-stranded DNA and emits red fluorescence under normal circumstances it cannot penetrate intact cell membranes However in late apoptotic and dead cells PI penetrates the cellular membrane staining the nuclei Dual staining with Annexin V-HF647 and PI enables clear differentiation between early apoptotic late apoptotic and dead cells
ILEX LIFE SCIENCES LLC EDTA P10-15100 6X100ML
PAN-Biotech EDTA 1:5000 (1X), 0.2 g/L in PBS, w/o Ca and Mg (6 x 100 ml). DESCRIPTION: PAN-Biotech EDTA 1:5000 (1X), 0.2 g/L in PBS, w/o Ca and Mg. Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often used to remove calcium from cell washing and suspension media. EDTA can reduce clumping of cells grown in liquid suspension. It is also used for gentle detachment of certain adherent cells for passaging. EDTA is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. EDTA: 0.02% EDTA. STERILE: Yes. STORAGE TEMP: +2-8°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
ILEX LIFE SCIENCES LLC EDTA 1P P10-026100 6 X 100 ML
PAN-Biotech EDTA 1% in PBS, w/o: Ca and Mg (6 x 100 ml). DESCRIPTION: PAN-Biotech EDTA 1% in PBS, w/o: Ca and Mg. Ethylenediaminetetraacetic acid (EDTA), a chelating agent, is often used to remove calcium from cell washing and suspension media. EDTA can reduce clumping of cells grown in liquid suspension. It is also used for gentle detachment of certain adherent cells for passaging. EDTA is often added to trypsin solutions to enhance enzymatic activity by neutralizing calcium and magnesium ions that enhance cell-to-cell adhesion and obscure the peptide bonds on which trypsin acts. LIQUID/POWDER: Liquid. PHENOL RED: Without Phenol Red. Ca/Mg CONTENT: Without Ca and Mg. EDTA: 1.00% EDTA. STERILE: Yes. STORAGE TEMP: +2-8°C. MANUFACTURER: PAN-Biotech GmbH (Germany).
Ethos Biosciences Buffer Diluent phosphate concentrate 1/EA
Dilurex 10X PBS is a concentrated solution of phosphate buffered saline intended as a stock solution Save space by keeping 10x concentrate and dilute to 1x pH 7 4 working concentration when needed by mixing 1 part 10X PBS with 9 parts distilled water Skip the tedium of weighing powders and stocking raw materials that can expire Our PBS is convenient ready to use and will save you hours in the lab letting you focus on breakthroughs rather than buffer prep Thousands of researchers trust Ethos Biosciences 10X PBS for its proven track record in top-tier publications and protocols - upgrade your productivity today