Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Research Products International Corp TES, Sodium Salt, 25 G
TES (N-[Tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid) Sodium Salt is a commonly used buffering agent in life science research. It helps to maintain a stable pH environment in biological experiments, such as cell culture, protein purification, and enzyme assays.
TES Sodium Salt is advantageous due to its ability to resist changes in pH over a wide range of temperatures, making it suitable for various experimental conditions.
Additionally, its low UV absorbance and minimal interference with biological molecules make it a preferred choice in many biochemical applications
National Diagnostics 1 liter size, TAE Buffer (50X) 0.2 micron filtration
Buffer; National Diagnostics; TAE (50X); Formulated w/18 Megohm water and 0.2u filtration; Saves time and improves results; Certified RNase and DNase free; Size: 1L
![Research Products International Corp RPI TBE 10X Solution [TRIS-borate-EDTA 10X Solution], 10 Liters](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-T32020-10000.0.JPG-250.jpg)
Research Products International Corp RPI TBE 10X Solution [TRIS-borate-EDTA 10X Solution], 10 Liters
TBE is a common buffer used in gel electrophoresis.
Supplied as a 1X concentrated solution containing:
89mM Boric Acid
2mM EDTA disodium salt
pH (1X solution @ 25°:C): 8.3+0.1
Research Products International Corp Tris-Glycine-SDS, 10 L
Common buffer used for running SDS-PAGE gels in electrophoresis.
1X Solution Contains:
TRIS/TRIS HCl 25 mM
Glycine 0.192 M
SDS 0.1%(w/v)
Sigma Aldrich Fine Chemicals Biosciences Phosphate Buffered Saline1L
Phosphate Buffered Saline (PBS) is routinely used as a wash buffer in Western blot and immunoprecipitation (IP) procedures as well as during sample preparation of cell lysates for use in general molecular biology applications.
Sigma Aldrich Fine Chemicals Biosciences HEPES hemisodium salt dry powder | 103404-87-1 | 10PAK
HEPES hemisodium salt dry powder | Mol Wt: 249.3 | 103404-87-1 | 10PAK
Sigma Aldrich Fine Chemicals Biosciences HEPES hemisodium salt >=99% (titration) | 103404-87-1 | 100G
HEPES hemisodium salt >=99% (titration) | Purity: >=99% (titration) | Mol Wt: 249.3 | 103404-87-1 | 100G
Sigma Aldrich Fine Chemicals Biosciences MOPSO sodium salt 99 titra500G
MOPSO sodium salt 99 titra500G
Sigma Aldrich Fine Chemicals Biosciences BIS TRIS propane BioXtra 9100G
BIS TRIS propane BioXtra 9100G
Sigma Aldrich Fine Chemicals Biosciences HEPES hemisodium salt | 103404-87-1 | | 25g
HEPES hemisodium salt | Purity: 99% | MW: 249.3 | 103404-87-1 | 25g
Sigma Aldrich Fine Chemicals Biosciences Hanks Balanced Salts Modif1L
HANKS Hanks Balanced Salts Modif1L
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
DULBECCOS Dulbeccos Modified Eagles1L